Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro
Siritantikorn S., Jintaworn S., Noisakran S., Suputtamongkol Y., Paris DH., Blacksell SD.
The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r = 0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-based methodology is cheaper, more standardised and better reproducible than a manual-based approach. © 2012 Royal Society of Tropical Medicine and Hygiene.