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Burkholderia pseudomallei causes melioidosis, a serious and often fatal bacterial infection. B. pseudomallei can behave as a facultatively intracellular organism and this ability may be important in the pathogenesis of both acute and chronic infection. The uptake of B. pseudomallei and other Burkholderia spp. by cells in tissue culture was examined by electron microscopy. B. pseudomallei can invade cultured cell lines including phagocytic lines such as RAW264, J774 and U937, and non-phagocytic lines such as CaCO-2, Hep2, HeLa, L929, McCoy, Vero and CHO. Uptake was followed by the intracellular multiplication of B. pseudomallei and the induction of cell fusion and multinucleate giant cell formation. Similar effects were produced by B. mallei and B. thailandensis.

Type

Journal article

Journal

Microbios

Publication Date

01/1998

Volume

96

Pages

71 - 93

Addresses

London School of Hygiene and Tropical Medicine, Great Britain.

Keywords

Caco-2 Cells, Hela Cells, U937 Cells, CHO Cells, Vero Cells, Intracellular Fluid, Eukaryotic Cells, Giant Cells, Animals, Cercopithecus aethiops, Humans, Burkholderia, Burkholderia pseudomallei, Culture Techniques, Cricetinae