Effectiveness of a Simplified Method for Isolation of Burkholderia pseudomallei from Soil
Limmathurotsakul D., Wuthiekanun V., Amornchai P., Wongsuwan G., Day NPJ., Peacock SJ.
ABSTRACT Detection of environmental Burkholderia pseudomallei indicates a risk for melioidosis and is important for the development of a global risk map. We describe a simple method for detecting B. pseudomallei using direct culture of soil in enrichment broth. This gives a rate of positivity comparable to that obtained with a standard method but is cheaper and labor saving.