Development of a dengue virus serotype-specific non-structural protein 1 capture immunochromatography method
Poltep K., Nakayama EE., Sasaki T., Kurosu T., Takashima Y., Phadungsombat J., Kosoltanapiwat N., Hanboonkunupakarn B., Suwanpakdee S., Imad HA., Srimark N., Kitamura C., Yamanaka A., Okubo A., Shioda T., Leaungwutiwong P.
Four serotypes of dengue virus (DENV), type 1 to 4 (DENV-1 to DENV-4), exhibit ap-proximately 25–40% of the difference in the encoded amino acid residues of viral proteins. Reverse transcription of RNA extracted from specimens followed by PCR amplification is the current stan-dard method of DENV serotype determination. However, since this method is time-consuming, rapid detection systems are desirable. We established several mouse monoclonal antibodies directed against DENV non-structural protein 1 and integrated them into rapid DENV detection systems. We successfully developed serotype-specific immunochromatography systems for all four DENV serotypes. Each system can detect 104 copies/mL in 15 min using laboratory and clinical isolates of DENV. No cross-reaction between DENV serotypes was observed in these DENV isolates. We also confirmed that there was no cross-reaction with chikungunya, Japanese encephalitis, Sindbis, and Zika viruses. Evaluation of these systems using serum from DENV-infected individuals indicated a serotype specificity of almost 100%. These assay systems could accelerate both DENV infection diagnosis and epidemiologic studies in DENV-endemic areas.