Detection of diverse Wolbachia 16S rRNA sequences at low titers from malaria vectors in Kayin state, Myanmar

Sawasdichai S., Chaumeau V., Dah T., Kulabkeeree T., Kajeechiwa L., Phanaphadungtham M., Trakoolchengkaew M., Kittiphanakun P., Akararungrot Y., Oo K., Delmas G., White NJ., Nosten FH.

<ns4:p><ns4:bold>Background</ns4:bold> <ns4:bold>:</ns4:bold> Natural <ns4:italic>Wolbachia </ns4:italic>infections in malaria mosquitoes were recently reported in Africa, and negatively correlated with the development of <ns4:italic>Plasmodium falciparum</ns4:italic> in the vectors. The occurrence and effects of <ns4:italic>Wolbachia </ns4:italic>infections outside Africa have not been described and may have been underestimated.</ns4:p><ns4:p> <ns4:bold>Methods</ns4:bold> <ns4:bold>:</ns4:bold> Mosquitoes were collected by human-landing catch during May and June 2017 in ten villages in Kayin state, Myanmar. Closely related species of malaria vectors were identified with molecular assays. 16S rRNA <ns4:italic>Wolbachia</ns4:italic> DNA sequences were detected with quantitative real-time PCR.</ns4:p><ns4:p> <ns4:bold>Results:</ns4:bold> Low titer of <ns4:italic>Wolbachia </ns4:italic>DNA was detected in 13/370 samples in six malaria vector species. Sequences were diverse and different from those described in the African malaria mosquitoes.</ns4:p><ns4:p> <ns4:bold>Conclusion:</ns4:bold> The detection of <ns4:italic>Wolbachia</ns4:italic> DNA in malaria mosquitoes from Kayin state warrants further investigations to understand better the ecology and biology of <ns4:italic>Anopheles</ns4:italic>-<ns4:italic>Wolbachia</ns4:italic> interactions in Southeast Asia.</ns4:p>

DOI

10.12688/wellcomeopenres.15005.4

Type

Journal article

Journal

Wellcome Open Research

Publisher

F1000 Research Ltd

Publication Date

05/11/2019

Volume

4

Pages

11 - 11

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